tobacco chewing and particularly spitting: Josey doesn't chew or spit in the beginning of the film, when he's ploughing with his son. He takes up this habit I guess as a way of showing us how, after loosing his family, he spits on everything/shows no respect towards anything anymore. He doesn't spit (although ready to) when first arriving to the farm. And he doesn't chew while branding the calf. He does take that habit up again when he wants to leave Laura Lee and the farm. It also shows with the dog. The first time he hits him on the forehead, and later he spits beside the animal.
music and music instruments: music symbolizes happiness and the feeling of being safe and being home. Laura Lee hides behind the piano and is only discovered when the piano is shattered. And the first time Josey allows himself any positive feeling towards the farm and Laura Lee, they are all dancing.
The Lost Lady: interesting name for a saloon... To me it symbolizes the point where he's gonna let go of the lady he's lost (his wife), and tackle a new life again (find the lost lady again - rebuild a new life)
There's plenty more, maybe I'll be back for some
